근관 충전용 Sealer가 수종의 세포에 미치는 독성효과에 관한 연구
A STUDY ON THE CYTOTOXICITY OF ROOT CANNAL SEALERS TO SEVERAL CELL LINES
임미경, 등원융이,
소속 상세정보
임미경 ( ) - 서울대학교
등원융이 ( ) - 서울대학교
KMID : 0362319920170020263
Abstract
This study was performed to evaluate and compare the cytotoxic effects of five root canal sealers to several different cell lines.
Five root canal sealers were AH-26, N2, Sealapex, Tubliseal, and Vitapex.
Each sealers were mixed according to the manufacturer's instructions, and culture media were added to each sealers immediately after mixing (the immediate group) and after three days (the third day group) and seven days (the seventh day group)
respectively.
And every sealer solutions were diluted to 1:1, 1:2, 1:3 and 1:4.
Three different permanent cell lines (HEp-2, McCoy, MRC-5) and human gingival fibroblasts and mononuclear cell were challenged by each sealer solution and the cytopathic effects were evaluated using MTT-ELISA, MTT-microscopy, and lactate
dehydrogenase
(LD) activity.
@ES The results were as follows:
@EN 1. In HEp-2 and MRC-5 cells, Vitapex was the least cytotoxic sealers.
2. AH-26 showed mild cytotoxic effects to HEp-2, gingival fibroblast and mononuclear cells.
3. N2 was the most toxic sealer to gingival fibroblast and it showed relatively strong cytotoxicity to HEp-2, McCoy and MRC-5 cells.
4. Tubliseal showed strong cytotoxic effects to HEp-2, McCoy, MRC-5, and mononuclear cells.
5. Sealapex showed strong cytotoxic effect to HEp-2, McCoy, and gingival fibroblasts.
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